$3,800.00
Clean and in excellent condition. Comes with MyiQ Real Time PCR System and laptop with IQ5 software. Working system from prominent DOE research facility.

Self test ran and passed. Standard cycle tested and ran the full test with no errors or exceptions. Heating and cooling was very prompt and all functions worked throughout the cycle.

Laptop and software run perfectly. PC connected successfully with system. We are not familiar enough with calibration and well mask settings to test further. 

Hassle-Free warranty.


Manufacturer Description:

Bio-Rad Offers Real-Time Instruments Designed for the Way You’d Rather Work

  • Modular instruments with high-resolution optics for maximum flexibility and sensitivity
  • Gradient functionality for rapid assay optimization
  • Outstanding thermal performance delivered by the iCycler® thermal cycler
  • PCR supermixes that provide optimum performance in real-time PCR assays
  • Precisely manufactured plates, tubes, and sealers tested for optimal fit and performance in Bio-Rad real-time PCR instruments

Smart Modular Designs

The MyiQ and iQ5 real-time PCR detection systems are 96-well modular upgrades to the iCycler thermal cycler, which delivers excellent thermal performance and thermal gradient capability. Sensitive charge-coupled device (CCD)-based optics enable accurate quantitation over a dynamic range of 9 orders of magnitude.

MyiQ System

The MyiQ system offers an affordable option for the detection of common green fluorescent dyes, such as FAM and SYBR Green I.

Optical system Excitation of all 96 wells is achieved by a combination of narrow-bandpass filters and a tungsten-halogen lamp. Filtered light from the lamp is reflected off mirrors, passes through a condensing lens, and is focused into the center of each well. Fluorescent light emitted from the wells reflects off the main fold mirror, passes through an emission filter, and is detected by a 12-bit CCD.

Excellent uniformity IL1-b plasmid template was diluted to 105 copies/reaction and amplified in the presence of a FAM-labeled detection probe with iQ™ supermix using an iQ5 real-time PCR detection system. Left panel, replicates of 10 μl reactions. Right panel, replicates of 25 μl reactions. Inset: CT, threshold cycle; SD, standard deviation.

Optimize Reactions in a Single Experiment

Bio-Rad real-time systems offer a thermal gradient feature, which allows you to optimize assays in a single experiment by using a range of temperatures simultaneously. At any step in a protocol, a temperature gradient of up to 25°C may be programmed across the reaction block.

  • Gradient control provides exceptional uniformity and reproducibility within each zone
  • Easy programming with onscreen presentation of gradient temperatures
  • Precision ramping
  • Available for both conventional and quantitative PCR instrumentation
  • Each temperature within the thermal gradient is listed in validation reports


Thermal gradient  
Optimizing incubation temperatures for real-time PCR assays is critical, particularly for multiplex assays, but it is not always easy to do. After the melting temperature (Tm) of a primer is calculated, the annealing temperature must be determined empirically for optimal results. This often involves repeating a reaction at many different temperatures. Similar time- consuming tests may be required to optimize the denaturation temperature. The thermal gradient feature facilitates identification of the most favorable temperatures for optimal assay performance.

Thermal gradient experiment for optimizing annealing temperature  A 10-fold dilution series (108 –102 copies) of plasmid containing GAPDH template was amplified using a thermal gradient in the presence of SYBR Green I dye. The amplification reaction was monitored and analyzed with an iQ5 real-time PCR detection system. Eight identical reactions were prepared for each template concentration, one for each of the eight PCR annealing temperatures, ranging from 55 to 70°C. An optimal primer annealing temperature of 58°C (shown in the green traces), which resulted in the earliest CT value, was identified in this gradient assay. The blue traces show the results for 64.5°C as a comparison.


Efficient, Accurate, Easy-to-Use Software

Making the most of a powerful real-time PCR system requires a flexible and easy-to-use software package. The iQ5 optical system software meets this need with quick-setup tools, a full suite of analysis tools, and a variety of presentation options. In addition, iQ5 optical system software, Security Edition, provides the tools required to meet regulatory requirements.

Standard features of iQ5 software include:

  • Advanced gene expression analysis options, including comparison to multiple reference genes, correction for reaction efficiency, and more
  • Ability to export any data collected and analyzed by iQ5 software directly to a Microsoft Excel spreadsheet
  • Gene Study module for multifile gene expression analysis to directly compare over 5,000 CT values from iQ5 and MyiQ data
  • User Profile management tool for creating unique user log-in names, with optional password protection, for file storage and data analysis preferences
  • Built-in analysis modules for absolute quantitation, melt-curve analysis, end-point analysis, and allelic discrimination (for multiplex data)


System Specifications

Temperature 
Range: 4–100°C
Accuracy: ±0.3°C of programmed target

Uniformity: ±0.4°C well-to-well


Gradient Mode

Gradient accuracy: ±0.4°C

Row uniformity: ±0.4°C

Gradient range: 40–100°C

Temperature differential range: 1–25°C


Optical System

-Light source: Tungsten-halogen lamp

-Fluorescence excitation range

--MyiQ: 475–495 nm

--iQ5: 475–645 nm

-Fluorescence detection range

--MyiQ: 515–545 nm

--iQ5: 515–700 nm

-Sensitivity
--One copy of IL-1
b in human genomic DNA
--Dynamic range: 9 orders of magnitude

Compatible dyes

--MyiQ: FAM, SYBR Green I

--iQ5: FAM, SYBR Green I, HEX, TAMRA, 
Texas Red, Cy5

Descriptive

Sample capacity: 96 wells

Sample volume: 15–100 μl

Dimensions (W x D x H): 29.2 x 58.4 x 38.7 cm (11.5 x 23 x 15.2")

Weight: 17.6 kg (39 lb)