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Manufacturer Description
Separa® Filter Vials and Accessories
Save time and money in the sample preparation process with the GVS Separa® Filter Vial.
Separa® is a one-step sample preparation device that allows a quick, effective, and simple to use process integrating a self-sampling system, membrane, cap, and lid with precut septa.
The liquid is placed in the outer vial and dispensed by pressing the inner insert so that it is pushed through the membrane. The sample is then filtered and Separa® is ready to be loaded into the autosampler.
Separa® Toggler 120
Is the multi compressor for simultaneous use up to 12 Separa®. It allows to save time and to reduce hand stress by filtering multiple Separa vials during sample preparation.
Properties
Separa® Filter Vial
- The device consists of an internal insert with a membrane chamber and precut septa cap and an external vial
- A sample ready to use after filtration
- Pre-slitted cap ensures easy and clean sample transfer
- Replace syringe, syringe filter, glass vial, and cap, reducing waste
- Increase sample integrity with all-in vial and filter
- Compatible with most auto-samplers
- Compatible with most multi-compressors
Applications
- Analytical Sample Prep, HPLC
- Analytical Sample Prep, uHPLC
- Biological Studies
- Chromatography
- Clarification
- Clarification of aqueous and organic solvent solutions
Development of a Simple DNA Extraction Method and Candida Pan Loop-Mediated Isothermal Amplification Assay for Diagnosis of Candidemia
To reduce the morbidity and mortality of candidemia patients through rapid treatment, the development of a simple, rapid molecular diagnostic method that is based on nucleic acid extraction and is superior to conventional methods for detecting Candida in the blood is necessary.
In the study, it was developed a multiplex Candida Pan/internal control (IC) loop-mediated isothermal amplification (LAMP) assay and a simple DNA extraction boiling protocol using Chelex-100 that could extract yeast DNA in blood within 20 min - the filtration process was performed using a SEPARA® tube (GVS, Bologna, Italy). The Chelex-100/boiling method for DNA extraction showed comparable efficiency to that of the commercial QIAamp UCP Pathogen Mini Kit using Candida albicans qPCR. In addition, the Candida Pan/IC LAMP assay showed superior sensitivity to that of general Candida Pan and species qPCRs against clinical DNA samples extracted with the QIAamp UCP Pathogen Mini Kit and Chelex-100/boiling method.
These results suggest that the Candida Pan/IC LAMP assay could be used as a rapid molecular diagnostic test for candidemia.
